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gltR [2017-12-15 17:11:34]
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gltR [2017-12-15 17:11:34]

transcriptional regulator (LysR family)
locus
BSU26670
pI
6.65
mw
32.79 kDa
protein length
296 aa Sequence Blast
gene length
888 bp Sequence Blast
function
unknown
product
transcriptional regulator (LysR family)
essential
no
synonyms
yrdL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

    • Gene

    Coordinates
    2,725,837 → 2,726,727

    Phenotypes of a mutant

  • The function of this protein is unknown. However, certain mutant forms are able to activate expression of the ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]'' operon (and thus to replace the cognate activator protein [protein|87BCAE725B02860156D50E1783F6DB68510C811E|GltC])

    • The protein

    Protein family

  • [SW|LysR family] of transcription factors

    • Additional information

  • The function of this protein is unknown. However, certain mutant forms are able to activate expression of the ''[gene|44DF1B7E476AD58B3CCC39300FFE0132D0D32AD0|gltA]-[gene|AA07CC52B2DD48ACC9D3375E9D531CB1ACBE485A|gltB]'' operon (and thus to replace the cognate activator protein [protein|87BCAE725B02860156D50E1783F6DB68510C811E|GltC])

    • Expression and Regulation

    Operons

    genes
    [gene|544CE1370BE7444F361F62808E320CE97C3C2F93|gltR]
    description
    (according to [http://dbtbs.hgc.jp/COG/prom/gltR.html DBTBS]) null

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9023181], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|544CE1370BE7444F361F62808E320CE97C3C2F93|GltR]: negative autoregulation, in [regulon|544CE1370BE7444F361F62808E320CE97C3C2F93|GltR regulon]
    • view in new tab

    Biological materials

    Mutant

  • GP345 (erm), available in the [SW|Stülke] lab
  • BKE26670 (Δ[gene|544CE1370BE7444F361F62808E320CE97C3C2F93|gltR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAGTCCTCCATAGTTG, downstream forward: _UP4_TAAATAAATGGATGGAAGGC
  • BKK26670 (Δ[gene|544CE1370BE7444F361F62808E320CE97C3C2F93|gltR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTAGTCCTCCATAGTTG, downstream forward: _UP4_TAAATAAATGGATGGAAGGC

    • Expression vector

  • pBP421 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP382]) (available in [SW|Fabian Commichau]'s lab)

    • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]

    • References

  • 9023181,28294562